Characterization of MRSA strains by phenotypic and OCR-based methods.
Characterization of MRSA strains by phenotypic and OCR-based methods.
Roum Arch Microbiol Immunol. 2016 Jan-Jun;75(1-2):37-43
Authors: Luncã C, Iancu LS, Vremerã T, Tuchiluş CG, Dorneanu O
Abstract
With the emergence and spread of new
methicilin resistant Staphylococcus aureus
(MRSA) strains, control of dissemination, both in
hospitals and in the community, requires the molecular
characterization of the circulating strains
in order to establish their dynamics and identify
the sources of infection. During this study we analyzed
the MRSA isolates by means of PCR-based
methods in order to improve epidemiological
surveillance and early application of prevention
measures. The presence of mecA, nuc, lukF-PV
and lukS-PV genes, as well as SCCmec types was
assessed in relation to clinical characteristics and
multidrug resistance (MDR) for 86 MRSA isolates
and showed that 51% of MDR strains were carriers
of mobile genetic elements SCCmec IV and the
majority of non-MDR SCCmec type IV strains
were PVL-positive (81.8%). Comparison of diagnostic
methods showed that PBP2 detection represents
an extremely useful alternative to PCR for
the rapid screening of MRSA isolates, in laboratories
that lack facilities necessary for molecular
diagnosis, such as PFGE (Pulse Field Gel Electrophoresis),
spa-typing and/or MLST (Multilocus
Sequence Typing).
PMID: 29616532 [PubMed - in process]
Source: Roumanian Archives of Microbiology and Immunology - Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
More News: Allergy & Immunology | Epidemiology | Genetics | Hospitals | Infectious Diseases | Microbiology | MRSA | Multidrug Resistance | Staphylococcus Aureus | Study | Superbugs