Polyploidization on SK-N-MC human neuroblastoma cells infected with herpes simplex virus 1.
We examined the impact of herpes simplex
virus-1 (HSV-1) on SK-N-MC human neuroblastoma
cell line.
The infected cells were followed from 6 hours up
to 96 hours post infection (hpi). A large number of
polyploid cells with giant nuclei was observed under
the influence of HSV-1 at 24 hpi with the DNA
content of 32c to 64c or more, in comparison with
control SK-N-MC cells that were characterized by
relatively moderate values of ploidy, i.e. 8с to 16с
(where 1c is the haploid amount of nuclear DNA
found in normal diploid populations in G0/G1). After
48-96 hpi, the population of polyploid cells with giant
nuclei decreased to the benchmark level. The SK-NMC
cells infected with HSV-1 for 24 hours were
stained with gallocyanine and monitored for
cytological features.
The infected cells underwent virus induced cellcell
and nuclei fusion with the formation of dense
nuclei syncytium. The metabolic activity of HSV-1
infected cells was higher in both nuclei and nucleoli
when compared to control cells.
PMID: 29616533 [PubMed - in process]
Source: Roumanian Archives of Microbiology and Immunology - Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
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