Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Nature 556, 7699 (2018). doi:10.1038/nature26155

Authors: Johnny H. Hu, Shannon M. Miller, Maarten H. Geurts, Weixin Tang, Liwei Chen, Ning Sun, Christina M. Zeina, Xue Gao, Holly A. Rees, Zhi Lin & David R. Liu
A key limitation of the use of the CRISPR–Cas9 system for genome editing and other applications is the requirement that a protospacer adjacent motif (PAM) be present at the target site. For the most commonly used Cas9 from Streptococcus pyogenes (SpCas9), the

Source: Nature - February 28, 2018 Category: Research Authors: Johnny H. Hu Shannon M. Miller Maarten H. Geurts Weixin Tang Liwei Chen Ning Sun Christina M. Zeina Xue Gao Holly A. Rees Zhi Lin David R. Liu Tags: Article Source Type: research