High-resolution definition of humoral immune response correlates of effective immunity against HIV

We report advances in a humoral immunoprofiling approach and its application to elucidate hallmarks of effective HIV-1 viral control. Systematic serological analysis for a cohort of HIV-infected subjects with varying viral control was conducted using both a high-resolution, high-throughput biophysical antibody profiling approach, providing unbiased dissection of the humoral response, along with functional antibody assays, characterizing antibody-directed effector functions such as complement fixation and phagocytosis that are central to protective immunity. Profiles of subjects with varying viral control were computationally analyzed and modeled in order to deconvolute relationships among IgG Fab properties, Fc characteristics, and effector functions and to identify humoral correlates of potent antiviral antibody-directed effector activity and effective viral suppression. The resulting models reveal multifaceted and coordinated contributions of polyclonal antibodies to diverse antiviral responses, and suggest key biophysical features predictive of viral control.

Source: Molecular Systems Biology - March 26, 2018 Category: Molecular Biology Authors: Alter, G., Dowell, K. G., Brown, E. P., Suscovich, T. J., Mikhailova, A., Mahan, A. E., Walker, B. D., Nimmerjahn, F., Bailey-Kellogg, C., Ackerman, M. E. Tags: Genome-Scale & Integrative Biology, Immunology, Systems Medicine Articles Source Type: research