Polymerase Bypass of N7-Guanine Monoadducts of Cisplatin, Diepoxybutane, and Epichlorohydrin

Publication date: Available online 20 March 2018 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Jiayu Ye, Caitlin R. Farrington, Julie T. Millard DNA oligonucleotides containing site-specific N7-guanine monoadducts of cisplatin, diepoxybutane, and epichlorohydrin were used as templates for DNA synthesis by two bacterial DNA polymerases and human polymerase β. These polymerases were able to bypass the lesions effectively, although the efficiency was decreased, with inhibition increasing with the size of the lesion. Fidelity of incorporation was essentially unaltered, suggesting that N7-guanine monoadducts do not significantly contribute to the mutational spectra of these agents.

Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 21, 2018 Category: Cytology Source Type: research