Study on Tissue Distribution of A Variety of Endogenous Metabolites By Air Flow Assisted Ionization-Ultra High Resolution Mass Spectrometry Imaging

Publication date: March 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 3 Author(s): WANG Zhong-Hua, HE Bing-Shu, SUN Cheng-Long, SONG Xiao-Wei, HE Jiu-Ming, ZHANG Rui-Ping, ABLIZ Zeper As a promising new molecular imaging technique, mass spectrometry imaging (MSI) has attracted more and more attention in the field of biomedicine. A method of air flow assisted ionization-ultra high resolution mass spectrometry-based mass spectrometric imaging (AFAI-MSI) was developed to profile endogenous metabolites in rat kidney tissue in this study. Rat kidneys were collected and cut into frozen tissue sections, and then were analyzed on an AFAI-MSI system operated in positive ion mode using acetonitrile- isopropanol-water (4:4:2, V/V, 5 μL min−1) as spray solvent, nitrogen gas as spray gas (0.6 MPa) and air as assisting gas (45 L min−1). The mass range and resolution were set to be 70–1000 Da and 70000, respectively. As a result, a total of 38 metabolites, ranging from 103 to 107 in signal strength and belonging to different metabolite types, including organic amines, sugars, vitamins, peptides, neurotransmitters, organic acids, phospholipids, sphingolipids, glycerides, and cholesterol esters, were identified and imaged to characterize their tissue-specific distribution in kidney tissues. Some metabolites, such as choline, acetycholine, betaine, phosphocholine, and glycerolphosphocholin were found to have distinct distribution along the cortex-medulla...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research