Rapid Characterization of Triacylglycerols in Edible Oils by Matrix-assisted Laser Desorption/Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Publication date: March 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 3 Author(s): LI Wei-Feng, YANG Qiu-Xia, LIN Ze-Peng, WANG Li-Ping, GUO Peng-Ran Composition and types of triacylglycerols (TAGs) in edible oils are closely related to our health. It is of vital significance to develop fast and high-efficiency methods for determination of TAGs. In this manuscript, qualitative analysis of TAGs was demonstrated using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS). 2,5-Dihydroxybenzoic acid (DHB) was employed as matrix and dichloromethane was used as solvent for dissolving the edible oils. With laser power of 15%, laser frequency of 100 Hz, and 100 laser shots, relative standard deviation (RSD) less than 9% was obtained. Different kinds of edible oils could be directly distinguished from each other using MS and tandem MS (MS/MS) results. With confidence level of 95%, principal component analysis (PCA) results showed that 34 different kinds of edible oils were clearly classified. Moreover, down to 5% of doped canola in olive was identified directly, indicating that MALDI-FTICR-MS has the potential for rapid analysis and screening of edible oils. Graphical abstract
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research