MicroRNA ‐320 involves in the cardioprotective effect of insulin against myocardial ischemia by targeting survivin

In this study, using a cell model of ischemia through culturing H9C2 cardiac myocytes in serum‐free medium with hypoxia, we demonstrated that pretreatment with insulin significantly inhibited cell apoptosis and downregulated microRNA‐320 (miR‐320) expression. Interestingly, miR‐320 mimic impaired the cardioprotective effect of insulin against myocardial ischemia injury by targeting survivin, which is a member of the family of inhibitor of apoptosis proteins. Suppression miR‐320 expression by miR‐320 inhibitor in H9C2 cells with myocardial ischemia mimics the cardioprotective effect of insulin by maintaining survivin expression. Taken together, miR‐320–mediated survivin expression involves in cardioprotective effect of insulin against myocardial ischemia injury.
Significance of this studyMyocardial ischemia/reperfusion (I/R) injury remains an important clinical problem with extremely deficient clinical therapies. Insulin exerts an antiapoptotic effect against I/R through the activation of PI3K/Akt/mTOR pathway. Here, we provided evidences to show that microRNA‐320 involves in the cardioprotective effect of insulin by targeting survivin, which is an inhibitor of apoptosis protein and functions as a key regulator in cell apoptosis and involves in the tumour genesis and progression. Our findings may provide a new potential therapeutic strategy for I/R injury and ischemic heart disease.

Source: Cell Biochemistry and Function - March 1, 2018 Category: Biochemistry Authors: Ni Yang, Liuzhong Wu, Ying Zhao, Ning Zou, Chunfeng Liu Tags: RESEARCH ARTICLE Source Type: research