Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TaqMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.

Source: Journal of Infection and Public Health - March 8, 2018 Category: International Medicine & Public Health Authors: Mehmet Demirci, Suat Saribas, Nigar Ozer, Sezer Toprak, Emel Caglar, Gonenc Ortakoylu, Pelin Yuksel, Gulsel Ayaz, Hrisi Bahar Tokman, Omer Uysal, Harika Oyku Dinc, Tevhide Ziver, Bekir Kocazeybek Source Type: research