5′‐coding sequence of the nasA gene of Azotobacter vinelandii is required for efficient expression

In this study, we continued expression analyses of the nasA gene and observed that the nasA 5′‐coding sequence plays an important role in gene expression, as demonstrated by the fact that deletions caused over sixfold reduction in the expression of the lacZ reporter gene. Further analysis suggests that the nasA 5′‐coding sequence promotes gene expression in a way that is not associated with weakened transcript folding around the translational initiation region or codon usage bias. The findings from this study imply that there exists potential to improve gene expression in A. vinelandii by optimizing 5′‐coding sequences.

A sequence element at the 5′ end of the nasA gene in Azotobacter vinelandii promotes gene expression.

Source: FEMS Microbiology Letters - September 5, 2014 Category: Microbiology Authors: Baomin Wang, Yumei Wang, Christina Kennedy Tags: Research Letter Source Type: research