Second ‐harmonic imaging microscopy for identifying colorectal intraepithelial neoplasia

In this study, second‐harmonic imaging microscopy was used to monitor precancerous colorectal lesions at different stages. It was found that the morphology of glands and lamina propria in mucosa changes with the progression of colorectal diseases from normal to low‐grade intraepithelial neoplasia to high‐grade intraepithelial neoplasia and this microscopy has the ability of direct visualization of these warning symptoms. Furthermore, two morphologic variables were quantified to determine the changes of glands and collagen in lamina propria during the development of colorectal intraepithelial neoplasia. These results suggest that second‐harmonic imaging microscopy has the potential in label‐freely and effectively distinguishing between normal and precancerous colorectal tissues, and will be helpful for early diagnosis and treatment of colorectal diseases. Lay Description Colorectal cancer is still a major public health issue in the world, and intraepithelial neoplasia has been widely regarded as a precancerous lesion. Early detection, accurate characterization, and resection of the precursor lesions are essential for the prevention of this malignancy. However, conventional endoscopy examination has limited ability to precisely identify colorectal intraepithelial neoplasia because of limited resolution. Thus, we try to use second‐harmonic imaging microscopy to monitor precancerous colorectal lesions at different stages, and then determine whether this microscopy ca...
Source: Journal of Microscopy - Category: Laboratory Medicine Authors: Tags: Original Article Source Type: research