Deciphering the biochemical and molecular mechanism underlying the in vitro and in vivo chemotherapeutic efficacy of ruthenium quercetin complex in colon cancer

In this study, the ruthenium quercetin complex has been synthesized and anticancer activity has been evaluated on a well‐defined model of DMH followed by DSS induced rat colon cancer and on human colon cancer cell line HT‐29. The characterizations accomplished through UV‐visible, NMR, IR, Mass spectra and XRD techniques, and antioxidant activity was assessed by DPPH, FRAP, and ABTS methods. In vitro study confirmed that the complex increased p53 expression, reduced VEGF and mTOR expression, apoptosis induction, and DNA fragmentation in the HT‐29 cells. Acute and subacute toxicity study was also assessed and results from in vivo study revealed that complex was efficient to suppress ACF multiplicity and hyperplastic lesions and elevated the CAT, SOD, and glutathione levels. Furthermore, the complex was found to decrease cell proliferation and increased apoptotic events in tumor cells correlates upregulation of p53 and Bax and downregulation of Bcl2 expression. Our findings from the in vitro and in vivo study support the continued investigation of ruthenium quercetin complex possesses a potential chemotherapeutic activity against colon cancer and was efficient in reducing ACF multiplicity, hyperplastic lesions in the colon tissues of rats by inducing apoptosis. Synthesis, characterization of ruthenium quercetin complex by UV, IR, NMR, Mass, XRD, and SEM. Free radical scavenging activity of quercetin was found to increase after complexation and the complex was found to i...
Source: Molecular Carcinogenesis - Category: Molecular Biology Authors: Tags: RESEARCH ARTICLE Source Type: research