Iron ‐sulfur protein maturation in Helicobacter pylori:Identifying a Nfu‐type cluster carrier protein and its iron‐sulfur protein targets

This article is protected by copyright. All rights reserved. Helicobacter pylori is anomalous among non‐nitrogen fixing bacteria in containing an incomplete NIF system for Fe‐S cluster assembly, comprised of NifS, NifU and a Nfu‐type protein. NifU‐depleted strains and Δnfu mutants were constructed and characterized. Recombinant Nfu was found to bind either one [2Fe‐2S] or [4Fe‐4S] cluster/dimer, based on analytical, UV‐visible absorption/CD and resonance Raman studies. A bacterial two‐hybrid system was used to ascertain interactions between Nfu, NifS, NifU, and each of 36 putative Fe‐S‐containing target proteins.
Source: Molecular Microbiology - Category: Microbiology Authors: Tags: Research Article Source Type: research