Bacterial attachment and biofilm formation on stainless steel surface and their in vitro inhibition by marine fungal extracts

This study evaluates anti‐biofilm activity of four extracts from marine fungi; Penicillium citrinum PR1T4, Sarocladium strictum PP2L4, Aspergillus sydowii PR3T13, and Aspergillus spp. PR5T4 against Staphylococcus aureus, Listeria monocytogenes (ATCC 19115), Escherichia coli, and Salmonella typhi (ATCC 14028). The ability of bacterial cells to adhere, detach, and form biofilm on stainless steel surface were examined and ethyl acetate extract of the fungal culture (15 mg/ml) were tested for anti‐biofilm activity for 3, 6, 9, 12, and 15 days. E. coli showed the highest ability to adhere (>8 log CFU/cm2) and lowest detachment (<4 log CFU/cm2) after 24, 48, and 72 hr. Extract PP2L4 had the highest anti‐biofilm activity against S. typhi (1.70 ± 0.04 log CFU/cm2). Fungal extracts, bacteria, and incubation period were significant factors and their interactions were significant. The results showed that marine fungal extracts are important natural sources for anti‐biofilm agents that have high potential as food‐contact surface sanitizers. Practical applicationsBiofilm removal from food contact surfaces has been one of the greatest challenges for food industry. There have been efforts to explore natural agents with anti‐biofilm properties. This study showed that marine‐derived fungal extracts significantly reduced the number of attached cell on stainless steel discs and, therefore, is potential candidates for anti‐biofilm agents. Special attention would be giv...
Source: Journal of Food Safety - Category: Food Science Authors: Tags: ORIGINAL ARTICLE Source Type: research