Transgenic mouse lines expressing the 3xFLAG ‐dCas9 protein for enChIP analysis

In this study, we generated transgenic mice expressing 3xFLAG‐tagged Streptococcus pyogenes dCas9 (3xFLAG‐dCas9) and retrovirally transduced gRNA into primary CD4+ T cells from these mice for enChIP. Using this approach, we achieved high yields of enChIP at the targeted genomic region. Our novel transgenic mouse lines provide a valuable tool for enChIP analysis in primary mouse cells.

In this study, we generated transgenic mice expressing 3xFLAG‐tagged Streptococcus pyogenes dCas9 (3xFLAG‐dCas9) and retrovirally transduced gRNA into primary CD4+ T cells from these mice for enChIP. Using this approach, we achieved high yields of enChIP at the targeted genomic region. Our novel transgenic mouse lines provide a valuable tool for enChIP analysis in primary mouse cells.

Source: Genes to Cells - February 26, 2018 Category: Genetics & Stem Cells Authors: Toshitsugu Fujita, Fusako Kitaura, Asami Oji, Naoki Tanigawa, Miyuki Yuno, Masahito Ikawa, Ichiro Taniuchi, Hodaka Fujii Tags: BRIEF REPORT Source Type: research