Entrapment of LTB protein in alginate nanoparticles protects against Enterotoxigenic Escherichia coli

In this study, feasibility of alginate nanoparticles (NPs) such as recombinant LTB from Enterotoxigenic Escherichia coli (ETEC) carrier was investigated. To do this, the eltb gene was cloned and expressed in E. coli BL21 (DE3) host cells, and a Ni‐NTA column purified the protein. NPs were achieved through ion gelation method in the presence of LTB protein and CaCl2 as the cross‐Linker and NPs were characterized physicochemically. Balb/C mice groups were immunized with LTB‐entrapped NPs or LTB with adjuvant and immunogenicity was assessed by evaluating IgG titer. Finally, the neutralization of antibodies was evaluated by GM1 binding and loop assays. LTB protein was expressed and efficiently entrapped into the alginate NPs. The size of NPs was less than 50 nm, and entrapment efficiency was 80%. Western blotting showed maintenance of the molecular weight and antigenicity of the released protein from NPs. Administration of LTB‐entrapped NPs stimulated antibody responses in immunized mice. Immunization induced protection against LT toxin of ETEC in ileal loops and inhibits enterotoxin binding to GM1‐gangliosides. Alginate NPs are also appropriate vehicle for antigen delivery purpose. Moreover because of their astonishing properties, they have the potential to serve as an adjuvant.

Source: APMIS - February 1, 2018 Category: Research Authors: Emad Kordbacheh, Shahram Nazarian, Abbas Hajizadeh, Davood Sadeghi Tags: Original Article Source Type: research