GM ‐CSF ameliorates microvascular barrier integrity via pericytes‐derived Ang‐1 in wound healing

Abstract Skin wound healing involves complex coordinated interactions of cells, tissues, and mediators. Maintaining microvascular barrier integrity is one of the key events for endothelial homeostasis during wound healing. Vasodilation is observed after vasoconstriction, which causes blood vessels to become porous, facilitates leukocyte infiltration and aids angiogenesis at the wound‐area, postinjury. Eventually, vessel integrity has to be reestablished for vascular maturation. Numerous studies have found that granulocyte macrophage colony‐stimulating factor (GM‐CSF) accelerates wound healing by inducing recruitment of repair cells into the injury area and releases of cytokines. However, whether GM‐CSF is involving in the maintaining of microvascular barrier integrity and the underlying mechanism remain still unclear. Aim of this study was to investigate the effects of GM‐CSF on modulation of microvascular permeability in wound healing and underlying mechanisms. Wound closure and microvascular leakage was investigated using a full‐thickness skin wound mouse model after GM‐CSF intervention. The endothelial permeability was measured by Evans blue assay in vivo and in vitro endothelium/pericyte co‐culture system using a FITC‐Dextran permeability assay. To identify the source of angiopoietin‐1 (Ang‐1), double staining is used in vivo and ELISA and qPCR are used in vitro. To determine the specific effect of Ang‐1 on GM‐CSF maintaining microvascular stabil...
Source: Wound Repair and Regeneration - Category: Surgery Authors: Tags: Original Research ‐Basic Science Source Type: research