Bacterial physiology: Spacers go off-site

Nature Reviews Microbiology 16, 122 (2018). doi:10.1038/nrmicro.2018.20 Author: Andrea Du Toit During the CRISPR–Cas adaptation process, the Cas1–Cas2 complex inserts new spacers between the leader sequence and the first repeat of the CRISPR array. Now, Nivala et al. report that non-canonical off-target integrations can occur in vivo at CRISPR repeat-like sequences within the genome
Source: Nature Reviews Microbiology - Category: Microbiology Authors: Tags: Research Highlight Source Type: research