In vitro and in vivo investigation of a novel amniotic ‐based chitosan dressing for wound healing

This study investigated the possibility of development of a novel amniotic‐based chitosan gel dressing as a potential wound repair substrate with marked efficacy. In this experiment, amniotic gel prepared based on chitosan/PVP gel containing human amniotic membrane extract (AME‐Gel) was investigated in terms of wound‐healing efficacy and scar preventive effects in a rat burn model. The levels of re‐epithelialization and dermal regeneration were examined by histological assessment using H&E and Masson's trichrome staining. Also, we clarified the mechanism of healing and cytokine‐releasing activities of AME as well as its effect on epithelization, angiogenesis and fibroblast growth and migration. Our results revealed that AME‐Gel induces epidermal and dermal regeneration at a shorter time through formation of granulation tissue, enhancement of fibroblast proliferation and improvement of blood capillary formation concomitant with developing collagen bundles. Therefore, AME‐Gel could be considered a simple and easy to be used as a biological dressing for any type of superficial burn wounds, without any adverse effects. This article is protected by copyright. All rights reserved.
Source: Wound Repair and Regeneration - Category: Surgery Authors: Tags: Original Research ‐Regeneration Science Source Type: research