Transformation of cellobiose during the interaction of cellobiose dehydrogenase and β‐glucosidase of Cerrena unicolor

This work investigated the regulatory role of the interaction between cellobiose dehydrogenase (CDH) and β‐glucosidase (β‐GLU) in the conversion of cellobiose into cellobionolactone or glucose in vitro. To study the regulation, the two enzymes were isolated from the culture medium of the fungus Cerrena unicolor grown on a medium with microcrystalline cellulose. The enzymes were obtained in an electrophoretically homogeneous state. Their properties were studied. Both enzymes had acidic pH optima and were more stable in the acidic pH range. CDH was moderately thermostable, while β‐GLU had a low thermostability. Both enzymes efficiently catalyzed the transformation of cellobiose. A mixture of CDH and β‐GLU transformed cellobiose to glucose or cellobionolactone in the presence of various concentrations of laccase and hydroquinone. Formation of glucose and cellobionolactone in vitro during the competition between CDH and β‐GLU for cellobiose depended on the availability of quinones, formed as a result of the interaction of laccase and hydroquinone, for CDH. At low laccase and hydroquinone concentrations, the formation of glucose was found to predominate over that of cellobionolactone. The possible physiological role of the enzymes’ interaction is discussed.

Source: Journal of Basic Microbiology - February 1, 2018 Category: Microbiology Authors: Alexander V. Lisov, Oksana V. Belova, Nataliya G. Vinokurova, Tatiana V. Semashko, Anatolii G. Lobanok, Alexey A. Leontievsky Tags: RESEARCH PAPER Source Type: research