High ‐sensitivity 5‐, 6‐, and 7‐color PNH WBC assays for both Canto II and Navios platforms

Conclusions: CD15‐gated PNH neutrophil clone size was quantified using either FLAER and CD157, FLAER and CD24, or CD157 and CD24. CD64‐gated PNH monocyte clone size was quantified using either FLAER and CD157, FLAER and CD14, or CD157 and CD14. Analysis of >40 PNH samples showed that the FLAER‐based plots derive virtually identical data to the non‐FLAER plot for neutrophils (R2=1) and monocytes (R2=0.9999) and that closely similar data can be acquired using both Canto II and Navios platforms with 7‐, 6‐ and 5‐color versions of the assay. Assessment of non‐PNH samples confirmed extremely low background rate of PNH phenotypes (neutrophils and monocytes) with all three approaches. This article is protected by copyright. All rights reserved.
Source: Cytometry Part B: Clinical Cytometry - Category: Molecular Biology Authors: Tags: Original Article Source Type: research