Utility of neutralization test for the laboratory diagnosis of suspected mumps cases

This study was performed to determine the diagnostic utility of a focus reduction neutralization test (FRNT) in comparison to MuV specific commercial IgM and IgG antibody enzyme immunoassays (EIA) in the suspected patients. One hundred‐eighty six samples collected from mumps outbreak during 2012‐16 were used for study. Samples (n=80) were tested by all the three serological assays, shows 70.4%, 83% and 92.5% positivity by IgM EIA, IgG and FRNT, respectively. Altogether 58.8% samples (n=47) were found positive in all three assays. Concordance between mumps RT‐PCR and IgM EIA is highest during first 2‐5 days and it decreases with increasing time post‐onset. Mumps FRNT results agree with those of RT‐PCR/IgM EIA from second week onwards, whereas the results of mumps IgG EIA agree with those of RT‐PCR/IgM EIA from 3‐10 post‐onset days. The results suggest the utility of a FRNT for the laboratory diagnosis of mumps cases from the unimmunized countries.
Source: Microbiology and Immunology - Category: Microbiology Authors: Tags: Original Article Source Type: research