Association of single nucleotide polymorphisms in WNT genes with the risk of Non ‐Syndromic Cleft Lip with or without Cleft Palate

Abstract Non‐syndromic cleft lip with or without palate (NSCL/P) is a common congenital deformity worldwide with multifaceted etiology. Interaction of genes and environmental factors have been indicated to be related with susceptibility to NSCL/P. Some WNT genes which are involved in craniofacial embryogenesis may play a key role in the pathogenesis of NSCL/P. In the present study we aimed to inspect the relationship between WNT3 (rs3809857, rs9890413), WNT3A (rs752107, rs3121310), and WNT10a rs201002930 (c.392 C>T) polymorphisms and NSCL/P in an Iranian population. The present case‐control study was carried out on 120 unrelated NSCL/P patients and 112 healthy subjects. The variants were genotyped by PCR‐RFLP method. The findings suggest that the rs3809857 polymorphism significantly decreased the risk of NSCL/P in codominant (OR=0.16, 95%CI=0.03‐0.75, p=0.020, TT vs GG), recessive (OR=0.16, 95%CI=0.03‐0.72, p=0.009, TT vs GG+GT) inheritance models. The rs9890413 variant marginally decreased the risk of NSCL/P in codominant (OR=0.41, 95%CI=0.17‐0.99, p=0.047, AG vs AA) model. Regarding C392T variant, the findings revealed that this variant significantly decreased the risk of NSCL/P in codominant (OR=0.24, 95%CI=0.10‐0.58, p=0.002, CT vs CC) and allele (OR=0.26, 95%CI=0.11‐0.62, p=0.002, T vs C) models. No significant association was observed between the rs752107 and rs3121310 variants and risk/protection of NSCL/P. Stratified analysis showed that WNT10a rs20...
Source: Congenital Anomalies - Category: Genetics & Stem Cells Authors: Tags: Original Article Source Type: research