Development of sea bream (Sparus aurata) semen vitrification protocols

The long-term goal of this research project is to set up efficient protocol that can be used to develop a standardized approach for vitrification of marine fish spermatozoa. In particular, the aim of the present study was to develop a vitrification protocol for sea bream (Sparus aurata) spermatozoa. To draw up the protocol, we tested two different dilution media (1% NaCl and Mounib medium), three different vitrification devices (loops, drops and cut straws), different cryoprotectants (CPs) and three different equilibration times (30, 60 and 120  s).

Source: Theriogenology - January 11, 2018 Category: Genetics & Stem Cells Authors: Loredana Zilli, Annalisa Bianchi, Maroua Sabbah, Laura Pecoraro, Roberta Schiavone, Sebastiano Vilella Source Type: research