Effect of superstimulation on the expression of microRNAs and genes involved in steroidogenesis and ovulation in Nelore cows

To better understand the impact of ovarian superstimulation on bovine follicular microenvironment, Nelore cows (Bos taurus indicus) were subjected to ovarian superstimulation with follicle stimulating hormone (FSH, n  = 10; P-36 protocol) or FSH combined with eCG (n = 10; P-36/eCG protocol). Follicular fluid was analyzed for cholesterol concentration. Granulosa cells were analyzed by RT-qPCR to assess the expression of genes involved in steroidogenic and ovulatory and expression of microRNAs involved in final follicular development and luteinizing hormone/choriogonadotropin receptor (LHCGR) expression.

Source: Theriogenology - January 11, 2018 Category: Genetics & Stem Cells Authors: P.H. Santos, R.A. Satrapa, P.K. Fontes, F.F. Franchi, E.M. Razza, F. Mani, M.F.G. Nogueira, C.M. Barros, A.C.S. Castilho Source Type: research