Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR – Cas9 genome-editing efficiency
We report the discovery of a genetically encoded inhibitor of 53BP1 that increases the efficiency of HDR-dependent genome editing in human and mouse cells. 53BP1 is a key regulator of DSB repair pathway choice in eukaryotic cells and functions to favor NHEJ over HDR by suppressing end resection, which is the rate-limiting step in the initiation of HDR. We screened an existing combinatorial library of engineered ubiquitin variants for inhibitors of 53BP1. Expression of one variant, named i53 (inhibitor of 53BP1), in human and mouse cells, blocked accumulation of 53BP1 at sites of DNA damage and improved gene targeting and chromosomal gene conversion with either double-stranded DNA or single-stranded oligonucleotide donors by up to 5.6-fold. Inhibition of 53BP1 is a robust method to increase efficiency of HDR-based precise genome editing.
Source: Nature Biotechnology - Category: Biotechnology Authors: Marella D Canny Nathalie Moatti Leo C K Wan Am é lie Fradet-Turcotte Danielle Krasner Pedro A Mateos-Gomez Michal Zimmermann Alexandre Orthwein Yu-Chi Juang Wei Zhang Sylvie M Noordermeer Eduardo Seclen Marcus D Wilson Andrew Vorobyov Meagan Munro Andrea Tags: Research Source Type: research