Evaluation of Sensititre Broth Microdilution Plate for Determining the Susceptibility of Carbapenem-resistant Klebsiella pneumoniae to Polymyxins

Colistin and polymyxin B MICs were determined for 106 carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates using Sensititre Research Use Only GNX2F plates (Thermo Fisher) and compared to CLSI broth macrodilution (BMD) as the reference method. For colistin, EUCAST breakpoints were applied and testing of isolates with very major (VM) errors was repeated in duplicate by both methods to determine a majority result. Essential agreement (MIC ± one dilution) of GNX2F with the reference method was 97.1% for polymyxin B and 92.5% for colistin (7 VM errors, 22.6%).

Source: Diagnostic Microbiology and Infectious Disease - January 9, 2018 Category: Microbiology Authors: Sandra S. Richter, James Karichu, Joshua Otiso, Hillary Van Heule, George Keller, Eric Cober, Laura J. Rojas, Andrea M. Hujer, Kristine M. Hujer, Steve Marshall, Frederico Perez, Susan D. Rudin, T. Nicholas Domitrovic, Keith S. Kaye, Robert Salata, David Source Type: research