A F örster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells

Publication date: January 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 1 Author(s): Min BAI, Xiao-Wen CAO, Feng CHEN, Yue ZHAO, Yong-Xi ZHAO Hydrogen sulfide (H2S) has been confirmed as a significant endogenous gaseous signaling molecule involved in various physiological processes. In order to monitor H2S in living cells, a Forster resonance energy transfer (FRET) ratiometric probe based on quantum dot-cresyl violet was developed. In this work, the quantum dot nanospheres via a facile ultrasonication emulsion strategy, and the mixture chloroform solution containing hydrophobic quantum dots and COOH-functionalized amphiphilic polymer were successfully transferred into the oil-in-water micelle. The negatively charged quantum dot nanospheres with quantum dots embedded in the polymer matrixes were successfully fabricated after the evaporation of chloroform. And then, these quantum dot nanospheres were condensed with positively charged cresyl violet-azide (CV-N3) via electrostatic interaction to obtain the complexes (QDS-N3). The as-prepared QDS-N3 complexes were monodispersed nanospheres with an average diameter of about 120 nm. These complexes were taken up by the cell through endocytosis, and they were still stable even in wide pH range. In addition, the QDS-N3 complexes exhibited no cellular toxicity which was verified by MTT assay. In this ratiometric probe, CV-N3 as a FRET acceptor was conjugated to quantum dot nanospheres. The quantum dots em...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research