Comparison of two commercial quantitative PCR assays and correlation with the first WHO International Standard for human CMV

Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard).Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP or MagNA Pure LC System followed by the kPCR PLXTM CMV DNA (kPCR) or the CMV R-geneTM assay (R-gene), respectively. 139 clinical specimens were tested. Both assays correlated well with the standard (R2>0.96) and a matrix effect was observed. Quantitative results correlated reasonably between both assays for whole blood (R2= 0.79) and well for other specimen types (R2= 0.93).

Source: Diagnostic Microbiology and Infectious Disease - January 7, 2018 Category: Microbiology Authors: Ilka Engelmann, Enagnon Kazali Alidjinou, Mouna Lazrek, Judith Ogiez, Jean-Marie Pouillaude, Emmanuel Chazard, Anny Dewilde, Didier Hober Source Type: research