Phytochemical screening and In-vitro antioxidant activity of < em > Centella asiatica < /em > extracts

Centella asiatica also known asmandukparnior Indian pennywort orjalbrahmi, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda.Centella asiatica has long been used to improve memory and cognitive function.The study aimed to identify the phytochemicals present in different solvent extracts ofCentella asiatica(i.e. PECA- Petroleum ether extract ofC. asiatica,CCA- Chloroform extract ofC. asiatica,EACA- Ethyl acetate extract ofC. asiatica, ECA- Ethanolic extract ofC. asiatica,HACA- Hydro-alcoholic extract ofC. asiatica)and evaluate the respective in-vitro antioxidant potentials.The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts ofCentella asiatica was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.In all different solvent extracts ofC. asiatica revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC50 values for ECA=128.752 ±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC50 values ECA=429.69 ±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity. The present study indicates that theCentella asiatica extracts ...
Source: International Journal of Phytomedicine - Category: Science Authors: Source Type: research