< em > In vitro < /em > antioxidant activities of stem bark, leaves and callus extracts from < em > Amoora rohituka < /em >

AbstractThe present work examined thein vitro antioxidant activity of sequentially extracted, solvent extracts (Petroleum ether, chloroform and Methanol) of stem bark, leaves and callus fromAmoora rohituka. The antioxidative capacity of the extracts were examined by using DPPH (1,1-diphenyl,2-picryl hydrazyl), ABTS (2,2 ’-azinobis-3-ethyl-benzothiozoline-6-sulphonic acid) and NOS (Nitric oxide scavenging) assays by using Gallic acid as standard control. Among the tested extracts, methanol extract of stem bark showed better antioxidative capacity. The total antioxidant activity was expressed as µg equivalent of G allic acid per gram on dry weight basis. IC50 values for methanol extract of stem bark were 7.53 µg/ml; 34.92 µg/ml and 56.31 µg/ml in ABTS, DPPH and NOS assays respectively. Whereas, leaves and callus extracts showed moderate antioxidative properties. 

Source: International Journal of Phytomedicine - December 27, 2017 Category: Science Authors: Kanthesh M Basalingappa Source Type: research