A seminested PCR assay for detection and typing of Human Papillomavirus based on E1 gene sequences

HPV infection is considered one of the leading causes of cervical cancer in the world. To date, more than 180 types of HPV have been described and viral typing is critical for defining the prognosis of cancer. In this work, a seminested PCR which allow fast and inexpensively detection and typing of HPV is presented. The system is based on the amplification of a variable length region within the viral gene E1, using three primers that anneal in potentially all HPV genomes. The amplicons produced in the first step can be identified by high resolution electrophoresis or direct sequencing.
Source: Diagnostic Microbiology and Infectious Disease - Category: Microbiology Authors: Source Type: research