Optimization and comparison of different techniques for complete extraction of saponins from T. terrestris

Publication date: Available online 19 December 2017 Source:Journal of Applied Research on Medicinal and Aromatic Plants Author(s): Boris Sarvin, Elena Stekolshchikova, Igor Rodin, Andrey Stavrianidi, Oleg Shpigun Ultrasound-assisted 1 1 UAE – ultrasound-assisted extraction. (UAE), refluxing 2 2 RE – refluxing extraction. (RE), low pressure refluxing 3 3 LPRE – low pressure refluxing extraction. (LPRE) and Soxhlet 4 4 SE – Soxhlet extraction. (SE) extraction techniques were developed for determination of steroidal saponins content in Tribulus terrestris L. Extraction parameters for UAE (solvent base: methanol, ethanol, isopropanol and acetonitrile; the ratio of solvent to soil: 50–400 mL/g; isopropanol and acetonitrile concentration: 30–100%; extraction time: 15–120 min), RE (solvent base: isopropanol and acetonitrile; the ratio of solvent to soil: 50–400 mL/g; isopropanol concentration: 30–80%) and SE (extraction time: 4, 8, 12, 18 and 24 h) methods were optimized by single-factor-experiment. Thermal decomposition of protodioscin was observed after 60 min of RE and 12 h of SE. Extraction time and temperature were optimized via double-factor-experiment for most promising RE and LPRE techniques. Comparison of UAE with RE, LPRE and SE methods was made for protodioscin and dioscin which are major polar and less polar T. terrestris saponins, correspondingly. The equal maximum of extraction yield was achieved using RE, LPRE and SE in optimiz...
Source: Journal of Applied Research on Medicinal and Aromatic Plants - Category: Complementary Medicine Source Type: research