Evaluation of consistency in quantification of gene copy number by real ‐time reverse transcription quantitative polymerase chain reaction and virus titer by plaque‐forming assay for human respiratory syncytial virus
In conclusion, RT‐qPCR measurement of RSV gene copy number was highly dependent on viral titer, and the detailed comparison of between each gene copy number and virus titer should be useful and supportive in confirming RSV plaque‐forming assay and virus dynamics. The technique may also be used to estimate the amount of RSV present in clinical specimens.
Source: Microbiology and Immunology - Category: Microbiology Authors: Keisuke Yamamoto, Noriko Ogasawara, Soh Yamamoto, Kenichi Takano, Tsukasa Shiraishi, Toyotaka Sato, Hiroyuki Tsutsumi, Tetsuo Himi, Shin ‐ichi Yokota Tags: Original Article Source Type: research