Detection of oral HPV infection – Comparison of two different specimen collection methods and two HPV detection methods

Very little is known about the natural history of oral HPV infection. Several different methods exist to collect oral specimens and detect HPV, but their respective performance characteristics are unknown. We compared two different methods for oral specimen collection (oral saline rinse and commercial saliva kit) from 96 individuals and then analyzed the samples for HPV by two different PCR detection methods (single GP5+/6+ PCR and nested MY09/11 and GP5+/6+ PCR). For the oral rinse samples, the oral HPV prevalence was 10.4% (GP+ PCR; 10% repeatability) vs 11.5% (nested PCR method; 100% repeatability).

Source: Diagnostic Microbiology and Infectious Disease - December 12, 2017 Category: Microbiology Authors: Marjorie M.A. de Souza, Gunter Hartel, David C. Whiteman, Annika Antonsson Source Type: research