Neonatal mouse cortical but not isogenic human astrocyte feeder layers enhance the functional maturation of induced pluripotent stem cell ‐derived neurons in culture
We present RNA‐sequencing data for an immature, fetal‐like status of our human iPS cell‐derived astrocytes as one possible explanation for their failure to enhance synaptic activity in our co‐culture system.
Main Points
Human iPS cell‐derived NSCs were differentiated to isogenic neurons or astrocytes.
Few neurons developed spontaneous activity.
Spontaneous activity was significantly increased by neuronal co‐culture on mouse but not isogenic human astrocytes.
Source: Glia - Category: Neurology Authors: Fritz W. Lischka, Anastasia Efthymiou, Qiong Zhou, Michael D. Nieves, Nikki M. McCormack, Matthew D. Wilkerson, Gauthaman Sukumar, Clifton L. Dalgard, Martin L. Doughty Tags: RESEARCH ARTICLE Source Type: research