Region ‐specific deletions of the glutamate transporter GLT1 differentially affect seizure activity and neurodegeneration in mice

In this study, we generated region‐specific GLT1 knockout mice by crossing floxed‐GLT1 mice with mice that express the Cre recombinase in a particular domain of the ventricular zone. Selective deletion of GLT1 in the diencephalon, brainstem and spinal cord is sufficient to reproduce the phenotypes (excess mortality, decreased body weight, and lethal spontaneous seizure) of the global GLT1 null mice. By contrast, dorsal forebrain‐specific GLT1 knockout mice showed nonlethal complex seizures including myoclonic jerks, hyperkinetic running, spasm and clonic convulsion via the activation of NMDA receptors during a limited period from P12 to P14 and selective neuronal death in cortical layer II/III and the hippocampus. Thus, GLT1 dysfunction in the dorsal forebrain is involved in the pathogenesis of infantile epilepsy and GLT1 in the diencephalon, brainstem and spinal cord may play a critical role in preventing seizure‐induced sudden death. Main Points GLT1 deletion in diencephalon, brainstem and spinal cord reproduces lethal seizures in global GLT1 KO mice. GLT1 deletion in dorsal forebrain causes transient nonlethal seizures and selective neuronal loss in cortical layer II/III and the hippocampus.
Source: Glia - Category: Neurology Authors: Tags: RESEARCH ARTICLE Source Type: research