Assessing the influence of isoflurane anesthesia on cardiac metabolism using hyperpolarized [1 ‐13C]pyruvate

Isoflurane is a frequently used anesthetic in small‐animal dissolution dynamic nuclear polarization‐magnetic resonance imaging (DNP‐MRI) studies. Although the literature suggests interactions with mitochondrial metabolism, the influence of the compound on cardiac metabolism has not been assessed systematically to date. In the present study, the impact of low versus high isoflurane concentration was examined in a crossover experiment in healthy rats. The results revealed that cardiac metabolism is modulated by isoflurane concentration, showing increased [1‐13C]lactate and reduced [13C]bicarbonate production during high isoflurane relative to low isoflurane dose [average differences: +16% [1‐13C]lactate/total myocardial carbon, –22% [13C]bicarbonate/total myocardial carbon; +51% [1‐13C]lactate/[13C]bicarbonate]. These findings emphasize that reproducible anesthesia is important when studying cardiac metabolism. As the depth of anesthesia is difficult to control in an experimental animal setting, careful study design is required to exclude confounding factors. Isoflurane, a commonly used anesthetic in small‐animal dynamic nuclear polarization‐magnetic resonance imaging (DNP‐MRI), is shown to have a dose‐dependent effect on the assessment of cardiac metabolism using [1‐13C]pyruvate in healthy rats. Experiments show an increased lactate and reduced bicarbonate production during high isoflurane dose relative to low dose. These findings underline the importa...
Source: NMR in Biomedicine - Category: Radiology Authors: Tags: RESEARCH ARTICLE Source Type: research