Impact of intratumoral heterogeneity of breast cancer tissue on quantitative metabolomics using high ‐resolution magic angle spinning 1H NMR spectroscopy

We describe the intratumoral variability of metabolite and lipid concentrations, quantified using high‐resolution magic angle spinning (HR MAS) 1H nuclear magnetic resonance (NMR) spectroscopy after multi‐core sampling of resected breast tumor tissue from three patients, and sampling of duplicate tissue cores from 15 additional breast tumors. In total, 32 metabolites were detected and quantified, and the intertumoral differences were, in general, found to be larger than the intratumoral differences, which suggests that one, or few, replicates per tumor are acceptable, supporting the feasibility of reliable cohort studies.

Source: NMR in Biomedicine - December 1, 2017 Category: Radiology Authors: Mikheil Gogiashvili, Salome Horsch, Rosemarie Marchan, Kathrin Gianmoena, Cristina Cadenas, Berno Tanner, Sabrina Naumann, Diana Ersova, Frank Lippek, J örg Rahnenführer, Jan T. Andersson, Roland Hergenröder, Jörg Lambert, Jan G. Hengstler, Karolina E Tags: RESEARCH ARTICLE Source Type: research