Small RNA-seq during acute maximal exercise reveal RNAs involved in vascular inflammation and cardiometabolic health: brief report
Exercise improves cardiometabolic and vascular function, although the mechanisms remain unclear. Our objective was to demonstrate the diversity of circulating extracellular RNA (ex-RNA) release during acute exercise in humans and its relevance to exercise-mediated benefits on vascular inflammation. We performed plasma small RNA sequencing in 26 individuals undergoing symptom-limited maximal treadmill exercise, with replication of our top candidate miRNA in a separate cohort of 59 individuals undergoing bicycle ergometry. We found changes in miRNAs and other ex-RNAs with exercise (e.g., Y RNAs and tRNAs) implicated in cardiovascular disease. In two independent cohorts of acute maximal exercise, we identified miR-181b-5p as a key ex-RNA increased in plasma after exercise, with validation in a separate cohort. In a mouse model of acute exercise, we found significant increases in miR-181b-5p expression in skeletal muscle after acute exercise in young (but not older) mice. Previous work revealed a strong role for miR-181b-5p in vascular inflammation in obesity, insulin resistance, sepsis, and cardiovascular disease. We conclude that circulating ex-RNAs were altered in plasma after acute exercise target pathways involved in inflammation, including miR-181b-5p. Further investigation into the role of known (e.g., miRNA) and novel (e.g., Y RNAs) RNAs is warranted to uncover new mechanisms of vascular inflammation on exercise-mediated benefits on health.
NEW & NOTEWORTHY How exerci...
Source: AJP: Heart and Circulatory Physiology - Category: Cardiology Authors: Shah, R., Yeri, A., Das, A., Courtright-Lim, A., Ziegler, O., Gervino, E., Ocel, J., Quintero- Pinzon, P., Wooster, L., Bailey, C. S., Tanriverdi, K., Beaulieu, L. M., Freedman, J. E., Ghiran, I., Lewis, G. D., Van Keuren-Jensen, K., Das, S. Tags: RESEARCH ARTICLE Source Type: research
More News: Cardiology | Cardiovascular | Eating Disorders & Weight Management | Heart | Insulin | Obesity | Physiology | Sports Medicine | Study