A real-time multiplex PCR for the identification and typing of Vibrio cholerae

We report the development and validation of a duo-triplex real-time PCR for the rapid identification and typing of Vibrio cholerae. The PCR assay targets a species specific toxR gene present in all strains of V. cholerae and used as a marker for the species, wbeO1 and wbfO139, encoding the O1 and O139 somatic antigens, and ctxA encoding CT. The two tcpA variants associated with the Classical and El-Tor biotypes are used to infer biotype. The assay was evaluated using 178 isolates comprising eight different Vibrio species, including 122 isolates of V.
Source: Diagnostic Microbiology and Infectious Disease - Category: Microbiology Authors: Source Type: research