Hot ‐water Extract of Sanguisorba officinalis Ameliorates Endotoxin‐induced Septic Shock by Inhibiting Inflammasome Activation

ABSTRACT The inflammasome is a multiprotein signaling complex that mediates inflammatory innate immune responses through caspase 1 activation and subsequent IL‐1β secretion. Nonetheless, its aberrant activation often leads to inflammatory diseases. Therefore, targeting the inflammasome holds promise for the treatment of inflammation‐related diseases. Our study showed that a hot‐water extract of Sanguisorba officinalis (HSO) effectively suppressed inflammasome activation triggered by ATP, nigericin, microbial pathogens, and dsDNA in bone marrow‐derived macrophages (BMDMs). HSO significantly suppressed IL‐1β production in a dose‐dependent manner; this effect correlated well with low levels of caspase 1 and ASC pyroptosome formation in HSO‐treated cells. This anti‐inflammatory activity of HSO was further confirmed in a mouse model of endotoxin‐induced septic shock. Oral administration of HSO reduced the level of IL‐1β in serum and in the peritoneal cavity, increasing the survival rate. Taken together, our results suggest that HSO is an effective inhibitor of inflammasome activation through NLRP3, NLRC4, and AIM2 pathways, and may be useful for treatment of inflammasome‐mediated diseases.
Source: Microbiology and Immunology - Category: Microbiology Authors: Tags: Original Article Source Type: research