Correcting CRISPR for copy number

Nature Genetics 49, 1674 (2017).
doi:10.1038/ng.3994

Authors: John Paul Shen & Trey Ideker
The CRISPR–Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

Source: Nature Genetics - November 29, 2017 Category: Genetics & Stem Cells Authors: John Paul Shen Trey Ideker Tags: News and Views Source Type: research