Evaluation of a human adenovirus viral load assay using the Altona RealStar ® PCR test

This study evaluated the performance of the Altona Diagnostics RealStar ® Adenovirus Research Use Only (RUO) real-time PCR reagents for HAdV quantitation in plasma samples from immunodeficient patients. The assay was linear from 2.30–9.17 log10 copies/mL (coefficient of determination; R2=0.998) with limits of detection and quantification of 2.19 log10 and 2.30 log10 copies/mL (>95% positivity rate), respectively. Assay precision was highly reproducible with coefficients of variance ranging from 0% to 4.7%.

Source: Diagnostic Microbiology and Infectious Disease - November 26, 2017 Category: Microbiology Authors: Hanna Rennert, Girish Ramrattan, Zhengming Chen, Patrick McIntire, Alber Michaeel, Anna Khazanova, Stephen G. Jenkins, John Sipley Source Type: research