Proteomic profiling of integral membrane proteins associated to pathogenicity in Vibrio parahaemolyticus strains

ABSTRACT Vibrio parahaemolyticus has been recognized as causal agent of the early mortality syndrome and is currently considered an emerging shrimp disease causing millionaire loses to aquaculture industry. Integral membrane proteins are widely recognized as pathogenicity factors involved in essential mechanisms which allow V. parahaemolyticus infection and make them attractive as therapeutic targets. However, physico‐chemical properties and low expression levels results usually into underrepresentation of these proteins in conventional bottom‐up proteomic, making integral membrane proteomics a challenging task. Integral membrane proteins from a bacterial strain isolated from early mortality syndrome infected white shrimp hepatopancreas and identified by 16S rRNA sequencing as V. parahaemolyticus and an ATCC strain pathogen for human, were obtained by a sequential extraction method and subjected to relative quantification and identification by iTRAQ. Homology database search allowed identification of more than two hundred proteins, whereas 35 proteins recognized as pathogenic factors showed statistically significant differential accumulation between both strains. Such proteins are mainly related to adherence, secretion systems, cell division, transport, lysogenization, movement and virulence. Identification of pathogenicity related proteins in V. parahaemolyticus represent valuable information for developing strategies based on molecular mechanisms that inhibit such prote...
Source: Microbiology and Immunology - Category: Microbiology Authors: Tags: Original Article Source Type: research