Efficient isolation of human metapneumovirus using MNT ‐1, a human malignant melanoma cell line with early and distinct cytopathic effects

In this study, it was demonstrated that, in the human malignant melanoma cell line MNT‐1, obvious syncytium formation occurs shortly after inoculation with HMPV‐positive clinical specimens. In addition, the growth and efficiency of isolation of HMPV were greater using MNT‐1 than using any other conventional cell line. Addition of this cell line to our routine viral isolation system for clinical specimens markedly enhanced isolation frequency, allowing isolation‐based surveillance. MNT‐1 has the potential to facilitate clinical and epidemiological studies of HMPV.

Source: Microbiology and Immunology - November 16, 2017 Category: Microbiology Authors: Ko Sato, Oshi Watanabe, Suguru Ohmiya, Fumiko Chiba, Akira Suzuki, Michiko Okamoto, Jiang Younghuang, Akihiro Hata, Hiroyuki Nonaka, Setsuko Kitaoka, Yukio Nagai, Kazuhisa Kawamura, Masahiro Hayashi, Satoru Kumaki, Tamio Suzuki, Kazuyoshi Kawakami, Hideka Tags: Original Article Source Type: research