Evaluation of oxidative stress in caprine bronchoalveolar lavage fluid of pneumonic and normal lungs

Publication date: Available online 9 November 2017 Source:International Journal of Veterinary Science and Medicine Author(s): T.A. Jarikre, G.O. Ohore, A.A. Oyagbemi, B.O. Emikpe Research in the area of oxidative stress in pneumonic pathology still requires attention in small ruminants especially with the use of bronchoalveolar lavage (BAL) which may be a more sensitive indicator of respiratory diseases than blood. This investigation evaluates the role of oxidative stressin the pathogenesis of caprine pneumonia using BAL fluid (BALf) from healthy and pneumonic goats. A BALf from 192 goats (whose pneumonic histopathology had been characterized using standard techniques) was biochemically assayed for anti-oxidants and pro-oxidants. Malondialdehyde (MDA), hydrogen peroxide generation (H2O2), myeloperoxidase (MPO) and reduced glutathione (GSH) contents were measured to assess free radical activity in the BALf. Superoxide dismutase (SOD), Glutathione transferase (GST) and Glutathione peroxidase (GPx) activity were also determined colourimetrically. There were significant increases in the BALf supernatant of MDA, H2O2 and MPO with decreases in GSH level and SOD activity in the pneumonic goats (P <0.05). There was also significant correlation of BALf oxidative assay to the type and severity of pneumonia. The levels of MDA, H2O2, and MPO increased significantly (P <0.05) in bronchopneumonia and bronchointerstitial pneumonia than other pneumonic conditions and norm...
Source: International Journal of Veterinary Science and Medicine - Category: Veterinary Research Source Type: research