Molecular characterization of virulence and antimicrobial resistance profile of Shigella species isolated from children with moderate to severe diarrhea in northeastern Brazil

Molecular characterization of virulence and antimicrobial resistance profiles were determined for Shigella species isolated from children with diarrhea in Fortaleza, Brazil. Fecal specimens were collected along with socioeconomic and clinical data from children with moderate to severe diarrhea requiring emergency care. Shigella spp. were isolated by standard microbiological techniques and we developed four multiplex polymerase chain reaction assays to detect 16 virulence-related genes (VRGs). Antimicrobial susceptibility tests were performed using disk diffusion assays.

Source: Diagnostic Microbiology and Infectious Disease - November 9, 2017 Category: Microbiology Authors: Pedro Henrique Quintela Soares de Medeiros, Aldo Ângelo Moreira Lima, Marjorie Moreira Guedes, Alexandre Havt, Mariana Duarte Bona, Luís Carlos Rey, Alberto Melo Soares, Richard Littleton Guerrant, Bernhard H. Weigl, Ila Fernanda Nunes Lima Source Type: research