Adenosine deaminase activity and gene expression patterns are altered after chronic ethanol exposure in zebrafish brain

Publication date: Available online 7 November 2017 Source:Neurotoxicology and Teratology Author(s): Eduardo Pacheco Rico, Denis Broock Rosemberg, Jotele Fontana Agostini Berteli, Andrei da Silveira Langoni, André Arigony Souto, Maurício Reis Bogo, Carla Denise Bonan, Diogo Onofre Souza Ethanol alters the homeostasis between excitatory and inhibitory neurotransmitters and its intoxication reveals adenosine as responsible to modify several responses including signal transduction. Zebrafish has been recently investigated for knowledge the prolonged effect of ethanol on behavioral and biochemical parameters. The aim of this study was to evaluate the soluble and membrane adenosine deaminase activities and gene expression in zebrafish brain. Animals were exposed to 0.5% ethanol for 7, 14, and 28days. There were no significant changes in ADA activity from soluble fraction after all treatments. However, we verified a decrease of ADA activity in membrane fraction after 28days (44%) of ethanol exposure. ADA1 was not altered whereas mRNA transcript levels for ADAL presented an increase after 28days of ethanol exposure (34%). ADA2-1 showed a decrease (26%) followed by an increase (17%) of transcripts after 14 and 28days of ethanol exposure, respectively. However, ADA2-1 truncated alternative splice isoform (ADA2-1/T) demonstrated a reduction after 28days (20%). ADA2-2 was decreased (22%) followed by an increase (109%) of transcripts after 14 and 18days of ethanol exposure, re...
Source: Neurotoxicology and Teratology - Category: Toxicology Source Type: research