Quantitation of Tolyporphins, Diverse Tetrapyrrole Secondary Metabolites with Chlorophyll ‐Like Absorption, from a Filamentous Cyanobacterium–Microbial Community

ConclusionsThe quantitative methods developed herein should facilitate investigation of the biosynthesis of tolyporphins (and other tetrapyrroles) as well as examination of other strains for production of tolyporphins. Copyright © 2017 John Wiley & Sons, Ltd.

Tolyporphins A–M, produced by a filamentous cyanobacterium (HT‐58‐2), absorb in the same spectral region as chlorophyll a. Fractionation by chromatography of large‐scale (440 L) cultures afforded isolated individual tolyporphins. Lipophilic extraction of small‐scale (2–50 mL) cultures, HPLC separation, and absorption detection enabled quantitation of tolyporphin A and chlorophyll a. Absorption spectroscopy with multicomponent analysis of lipophilic extracts afforded the ratio (up to 1.8‐fold) of all tolyporphins to chlorophyll a.

Source: Phytochemical Analysis - November 1, 2017 Category: Biochemistry Authors: Yunlong Zhang, Ran Zhang, Rebecca ‐Ayme Hughes, Jingqiu Dai, Joshua R. Gurr, Philip G. Williams, Eric S. Miller, Jonathan S. Lindsey Tags: Research Article Source Type: research